Plant Tissue Culture Ppt Pdf 95%

Use sections 1 through 8 as direct slide titles or chapter headings.

A greenhouse where the plantlets are moved from the lab to natural soil conditions. 3. Stages of Plant Tissue Culture

Genetic manipulation, such as genetic engineering (transformation) and soma-clonal variation selection. plant tissue culture ppt pdf

The rapid multiplication of plants (e.g., bananas, potatoes) to produce high-quality, disease-free clones.

The ability of plants to modify their metabolism, growth, and development to adapt to the environmental conditions (i.e., in vitro environment). 2. Basic Requirements for a Plant Tissue Culture Lab Use sections 1 through 8 as direct slide

: Choosing a healthy donor plant and preparing the explant (the piece of tissue used to start the culture).

(e.g., BAP, Kinetin, Zeatin): Promote cell division and shoot proliferation. The Morphogenetic Rule: High Auxin : Low Cytokinin →right arrow Rooting or Callus formation Low Auxin : High Cytokinin →right arrow Shooting (Shoot proliferation) Balanced Auxin : Cytokinin →right arrow Unorganized Callus Growth 5. Major Steps in Plant Tissue Culture Stages of Plant Tissue Culture Genetic manipulation, such

Transferring shoots to a medium rich in Auxins to develop a root system.

An unorganized, proliferating mass of actively dividing parenchymatous cells. Explants are placed on high-auxin media (often containing 2,4-D). Callus cultures are excellent for studying secondary metabolite production, inducing somaclonal variations, or initiating cell suspension cultures. Organ Culture